超低分子量蛋白Marker I（3.3-22 kD）（非預染）

	分離膠	濃縮膠
	18％T, 5%C /5 ml	5％T, 3.3%C/2 ml
40% PAA（19:1）	2.25 ml	/
40% PAA (29:1)	/	0.25 ml
4×凝膠緩沖液	1.25 ml	0.5 ml
乙二醇（電泳級）	1.5 ml	/
ddH₂O	/	1.25ml
10％APS	50 μl	20 μl
TEMED	5 μl	2 μl

II 配制濃縮膠

去除覆蓋在分離膠上的乙醇層，用濾紙將殘留乙醇吸去。

1．按照表一將不同體積的雙蒸水、40%PAA（29:1）和4×凝膠緩沖液加入到小燒杯中混合。

2．加入10%過(guò)硫酸銨和TEMED，立即混勻5-10秒，以使溶液充分混勻。

3．將梳子插入凝膠內，避免產(chǎn)生氣泡。

4．靜置30-60分鐘裝待凝膠聚合

二. 電泳：

1. 取Marker樣品，充分混勻后，95℃處理5分鐘。

2. 電泳前，將10×陽(yáng)極緩沖液（Cat No:AB080）和10×陰極緩沖液（Cat No:CB010）用蒸餾水稀釋成1×緩沖液備用。將電泳槽的外槽加入1×陽(yáng)極緩沖液，內槽加入1×陰極緩沖液，輕柔拔出梳子，將Marker或蛋白樣品（已經(jīng)過(guò)2×Tricine上樣緩沖液[Cat No:TP050]處理）加入點(diǎn)樣孔，參考下表進(jìn)行電泳，至每條帶都清晰可見(jiàn)時(shí)停止電泳。整個(gè)電泳過(guò)程大約需要2-3個(gè)小時(shí)。

表二 多肽電泳條件

恒電壓	150V
起始電流	60-75mA/板膠
結束電流	15-25mA/板膠
電泳時(shí)間	2.5-3小時(shí)

多肽電泳配套試劑

名稱(chēng)	貨號	規格
超低分子量蛋白電泳試劑盒	RTD6120	10次
Tris-Tricine-SDS-PAGE凝膠快速制備及電泳試劑盒（含預染Marker）	RTD6121	10次
Tris-Tricine-PAGE凝膠制備及電泳試劑盒（多肽變性電泳）	RTD6122	25次
超低分子量蛋白Marker I（3.3-22 kD）（非預染）	RTD6101	10次（100 μl）
超低分子量蛋白Marker III（3.4-100 kD）（非預染）	RTD6125	10次（50 μl）
超低分子量蛋白Marker V（3.5-42 kD）（非預染）	RTD6113	20次（100 μl）
彩虹預染超低分子量蛋白Marker（1.2-45 kD）	RTD6110	10次（50 μl）
彩虹預染超低分子量蛋白Marker II（3-40 kD）	RTD6145-01	10次（50 μl）
雙色預染超低分子量蛋白質(zhì)Marker（1.5-42 kD）	RTD6148	10次（50 μl）
40% PAA（19:1）	AC1914-01	100ml
40% PAA（29:1）	AC2914-01	100ml
49.5％ T 3％C PAA溶液	PS080-01	100ml
49.5％ T 6％C PAA溶液	PI080-01	100ml
4×多肽電泳凝膠緩沖液	GB010-100ml	100ml
2×Tricine 上樣緩沖液（變性，還原）	TP050	10×1ml
2×Tricine 上樣緩沖液（變性，還原，含溴酚藍）	TP080	10×1ml
FastBlue蛋白染色液	RTD6202-02	500ml
乙二醇（電泳級）	EA0582-02	100 ml
50%甘油（多肽電泳用）	GA1010-50ml	50 ml
10×Tris-Tricine-SDS緩沖液(變性電泳，粉末型)	CB010P	500 ml
10×陽(yáng)極緩沖液（多肽電泳用）	AB080	250 ml
10×陰極緩沖液（多肽電泳用）	CB010	100 ml

使用蛋白Marker產(chǎn)品發(fā)表部分文章列表：

1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.

Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Food Control 50 (2015) 896e900

Institution：Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper link：http://dx.doi.org/10.1016/j.foodcont.2014.10.040

2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by isolated from fermented sausage Lactobacillus plantarum.

Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

Zhang, Changqing Yu, Xue Han

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Food Control Volume 81, November 2017, Pages 211-217

Institution：Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper link：https://doi.org/10.1016/j.foodcont.2017.05.030

3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

Marker: RTD6110，彩虹超低分子量蛋白Marker（1.2-45 kD）

Journal: Frontiers in Plant Science. Volume 81, November 2017, Pages 211-217

Institution：Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper link：https://doi.org/10.1093/jxb/ery351

4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.

Author: Xinran Lv, Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li

Marker: RTD6127，超低分子量蛋白Marker IV（3.3-45 kD）

Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)

Institution：College of Biological Science and Biotechnology, Beijing Forestry University

Paper link：https://link.springer.com/article/10.1007/s00217-017-2958-3

5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

Marker: RTD6110，彩虹超低分子量蛋白Marker （1.2-45 kD）

Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019，613-626

Institution：Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper link：https://doi.org/10.1093/jxb/ery351

6. [2021 IF=11.277] An Alternative Splicing Variant of PtRD26 Delays Leaf Senescence by Regulating Multiple NAC Transcription Factors in Populus.

Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

Marker: RTD6110，彩虹預染超低分子量蛋白（1.2-45 kD）

Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

Institution：Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

Paper link：https://doi.org/10.1093/plcell/koab046

7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Author: Feng Qin, Shujuan Wang, Mingxia Gao and Xiangmin Zhang

Marker: RTD6110，彩虹預染超低分子量蛋白（1.2-45 kD）

Journal: Analytical Methods. 2021,13, 2804-2811

Institution：Department of Chemistry, Fudan University

Paper link：https://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.

Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng

Marker: RTD6142 高分子量非變性電泳蛋白質(zhì)Marker II（45-669 kD）

Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892

Institution：College of Veterinary Medicine, Hunan Agricultural University,

Paper link：https://linkinghub.elsevier.com/retrieve/pii/S1877959X21002454

9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger

Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia

Marker: RTD6109 高分子量蛋白質(zhì)Marker（43-200 kD）

Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632

Institution：College of Food Science and Technology, Hebei Normal University of Science and Technology

Paper link：https://link.springer.com/article/10.1134/S0003683810060116

10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.

Author: Dong Liu

Marker: RTD6103 低分子量蛋白質(zhì)Marker II（14.4-116 kD）

Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33

Institution：Department of Food Processing Technology, Anqing Vocational & Technical College

Paper link：https://doi.org/10.1111/1471-0307.12395

11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy

Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu

Marker: Protein Marker

Journal: Biomedical Materials, 2022 Jan 24;17(2)

Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

Paper link：https://pubmed.ncbi.nlm.nih.gov/34996052/

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