Anti-RbcL subunit of RuBisCO
antibody
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產(chǎn)品編號
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產(chǎn)品名稱(chēng)
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規格
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RGR2030
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Anti-plant RbcL (兔多抗)
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20 μl
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● Product Information
Ribulose-1,5-bisphosphate
carboxylase/oxygenase commonly known by the abbreviation RuBisCO, is an enzyme
involved in the first major step of carbon fixation, a process by which
atmospheric carbon dioxide is converted by plants to energy-rich molecules such
as glucose. In chemical terms, it catalyzes the carboxylation of
ribulose-1,5-bisphosphate (also known as RuBP). It is probably the most
abundant enzyme on Earth. The enzyme usually consists of two types of
protein subunit, called the large chain (RbcL) and the small chain (RbcS).
Description: Rabbit polyclonal antibody
Synonyms: RbcL, Ribulose-1,5-bisphosphate carboxylase, oxygenase
Immunogen: KLH-conjugated synthetic peptide of RbcL derived from Arabidopsis
thaliana ATCG00490.
Form: Liqiud
Purification: Immunogen affinity purified
Stability
&Storage: Use a manual defrost freezer and avoid
repeated freeze-thaw cycles. 12 months from date of receipt, -20 to-70℃as supplied.6 months, -20 to-70℃under sterile conditions after reconstitution. 1 month, 2 to8℃under sterile conditions after reconstitution.
Shipping: The product is shipped at4℃.
Upon receipt, store it immediately at the temperature recommended above.
● Application Information
Recommended Dilution: Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations
should be determined by the end user.
Expected/apparent MW: 53 / 53-55 kDa
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: Among 25 analyzed species, the sequence of the synthetic peptide used for
immunization is 100% homologues with the sequence in Leymus chinensis,
Oryza sativa Indica Group, Zea mays, Cucumis sativus, Gossypium raimondii,
Hordeum vulgare subsp. Vulgare, Medicago truncatula, Brassica napus, Solanum
tuberosum, Solanum lycopersicum, Nicotiana tabacum, Triticum aestivum, Panicum
virgatum, Sorghum bicolor, Vitis vinifera, Populus trichocarpa, Physcomitrella
patens, Spinacia oleracea, Chlamydomonas reinhardtii, Glycine max, Setaria
viridis
● Experimental Data
Lane
1: 5 μg soluble protein from Arabidopsis thaliana leaf.
Lane 2: 10 μg soluble protein from Arabidopsis
thaliana leaf.
Lane 3: 15 μg soluble protein from Arabidopsis
thaliana leaf.
Lane 4: 1.1 μg stromal protein from Arabidopsis
thaliana leaf.
Lane 5: 2.75 μg stromal protein from Arabidopsis
thaliana leaf.
Lane 6: 5.5 μg stromal protein from Arabidopsis
thaliana leaf.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or4℃for 1h.
Primary antibody: 1:2000 dilution overnight at4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L
(HRP) (Cat#HGR1020 )
Detection: using chemiluminescence substrate and image
were captured with CCD camera.
with CCD camera.